3,300,000
g/mol
0.0303
pmol
0.00003
nmol
30.3
fmol
18,248,484,848.48
3,300
ng/pmol
3,300,000
g/mol
0.0303
pmol
0.00003
nmol
30.3
fmol
18,248,484,848.48
3,300
ng/pmol
The DNA/RNA Unit Converter allows you to convert between micrograms (µg), picomoles (pmol), femtomoles (fmol), and number of molecules for nucleic acids. This is essential when setting up cloning reactions, transfections, or any experiment that requires precise molar quantities rather than mass measurements. The tool supports dsDNA, ssDNA, and ssRNA, each with the appropriate average molecular weight per base.
The converter uses the following relationships:
pmol = µg / (MW × 10⁻⁶)
Where MW = length × average Da per bp/nt.
fmol = pmol × 1000
Molecules = pmol × 6.022 × 10¹¹
The molecular weight depends on the type of nucleic acid:
Inputs
Results
1 µg of a 5 kb dsDNA plasmid corresponds to approximately 0.303 pmol or 303 fmol, which is about 1.82 × 10¹¹ molecules.
Inputs
Results
0.5 µg of a 20-nt ssDNA oligonucleotide is approximately 75.76 pmol, a very large number of molecules.
Many molecular biology reactions, such as ligations, depend on the molar ratio of insert to vector, not their mass ratio. Since a short insert fragment weighs much less per molecule than a large vector, using mass alone would result in incorrect stoichiometry. Molar quantities ensure the correct number of molecules are mixed.
1 pmol = 1000 fmol. Picomoles (pmol, 10⁻¹² mol) and femtomoles (fmol, 10⁻¹⁵ mol) are both sub-molar units. Femtomoles are used when dealing with very small quantities, such as trace amounts of DNA in forensic or single-cell analysis.
Use the reverse formula: µg = pmol × MW × 10⁻⁶. For example, 1 pmol of a 5 kb dsDNA (MW = 3,300,000 Da) is: 1 × 3,300,000 × 10⁻⁶ = 3.3 µg.
Roboculator Team
The Roboculator Team explains calculations, planning tools, and practical formulas in clear language for real-life situations.
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